SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500g

SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500gSD Broth 2% Glucose w o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. Suspend 25. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature

The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations

25 gram in 1 litre distilled water for 1X supplements

at this strength the ph is around 6

Weight 368

Yeast extract 5 Dextrose 30 Total 35

15 g SP supplements (Ade

YPD medium (YEPD) is a complex medium for routine growth

32 KH2PO4 870 Carbon Source   Glucose 10000 Total 19334

Lysine and Methionine

trace elements and salts resulting in a good development of yeast colonies

Tryptone 10 Yeast extract 5 NaCl 0

NH2CH2COCH2CH2COOH