SD Broth / 2% Glucose w/o Phosphate (KH2PO4)

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Description

SD Broth / 2% Glucose w/o Phosphate (KH2PO4)SD Broth 2% Glucose w o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. Suspend 25. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature

Tryptone 12 Yeast Extract 24 Final pH 7

20mM Glacial Acetic Acid and 1mM Na2EDTA

Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor

The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate

The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis

NH2CH2COCH2CH2COOH

0 Suspend 36

4 Riboflavin 0

5 Purity HPLC >99% Appearance White Powder Store dry at room temperature

These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies

minus Glutamic acid

Weight 112

Exchange/Return Notes